Interrogating Cell Mechanics with Spectroscopy Plus

This application note outlines the advancements in material characterization, specifically using Nanosurf’s Spectroscopy Plus Option within Nanosurf Studio for studying nanoscopic mechanical properties of materials. It emphasizes the use of atomic force microscopy (AFM) and introduces sophisticated techniques such as force-displacement (F-D) curves, force-time (F-T) curves, and oscillatory testing. These methods provide detailed insights into the viscoelastic properties of materials, particularly biological cells. The Spectroscopy Plus option enables users to customize experiments to probe material responses under various conditions, enhancing the capability to understand material behaviors like elasticity, viscosity, and energy dissipation.

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Learning Objectives

  1. Recognize the principles and applications of force-displacement and force-time curves in atomic force microscopy.
  2. Learn to analyze viscoelastic properties of materials using time-relaxation curves and understand models such as the Maxwell and Kelvin-Voight.
  3. Gain skills in using the Spectroscopy Plus option to design and conduct customized experiments, exploring complex material behaviors
  4. Understand the method and significance of oscillatory testing in determining the loss tangent of materials, indicating energy dissipation.
  5. Develop the ability to interpret and utilize data from various experimental setups to assess material properties under different loading conditions and histories.
  6. Learn to synchronize and integrate AFM with other instruments, expanding the scope and depth of experimental investigations in material science.
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